Human insulin receptor RFLPs detected by Hindlll and Dral
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TaqL and MspL RFLPs are detected by the human 2,3-biphosphoglycerate mutase (BPGM) cDNA.
Source/Description: The XMEL4 clone is a 1.5 kb cDNA subcloned into the EcoRI site of pBluescript SKII +. XMEIA was isolated from a human melanoma cDNA library. Sequence analysis showed complete homology between XMEL4 and the 2,3-biphosphoglycerate mutase (BPGM) cDNA sequence (1, 2). Polymorphism: Both Taql and Mspl detect two-allele polymorphisms when probed with XMEIA Frequency: Allele freque...
متن کاملThree RFLPs for the insulin receptor gene INSR: EcoRI, Pst I, Hind III.
SOURCE AND DESCRIPTION OF CLONE: A 973 bp cDNA insert from an adult human kidney library cloned in the EcoRI site of PBR 327 (pINSR 18.2) encoding aminoacids-26 to 295 of the insulin receptor. POLYMORPHISM EcoRI identifies a two allele polymorphism with bands at 22 fcb and 20 Kb Hind III detects two allelic fragments of 6.5 Kb and 1.4 kb Pst I reveals two allelic fragments of 2.
متن کاملA frequent Hindlll RFLP of the human fibrorrertin gene ( FNl )
SOURCE/DESCRIPTION: The probe used is a Hindlll/BamHI fragment of 1.4kb from the fibronectin recombinant, pFH6 (Kornblihtt, A.R. et al.). POLYMORPHISM: Hindlll (A/AGCTT) (New England, Bio-Labs, FRG) detects invariant bands at 4.75 and 1.7kb in addition to a simple two-allele polymorphism defined by the presence of a band of 7.1kb (F2) alternatively with two bands of 3.7 and 3.4kb (Fl) (Figure 1...
متن کاملMultiple alleles for tuber shape in diploid potato detected by qualitative and quantitative genetic analysis using RFLPs.
Tuber shape in potato is commonly regarded as displaying continuous variation, yet at the diploid level phenotypes can be discerned visually, having round or long tubers. Inheritance of qualitative tuber shape can be explained by a single locus Ro, round being dominant to long. With restriction fragment length polymorphisms (RFLPs) the Ro locus was mapped on chromosome 10. Tuber shape was also ...
متن کاملVariations of the Normal Human Limbal Stem Cells Detected by In Vivo Confocal Microscopy
Background To report normal variations of the limbal structures using in vivo laser scanning confocal microscopy. Methods: This was a retrospective study of fourteen eyes from 11 healthy individuals. Confocal imaging of cornea and limbus was performed using Heidelberg Retina Tomograph III Rostock Corneal Module. Results: The typical structure of the palisades of Vogt (POV) was detected ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.3.1277